Challenges of “Sticky” Co-immunoprecipitation: Polyalanine Tract Protein–Protein Interactions

Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.

Source: Springer protocols feed by Genetics/Genomics - June 3, 2013 Category: Genetics & Stem Cells Source Type: news