Endophytic Bacillus megaterium BM18-2 mutated for cadmium accumulation and improving plant growth in Hybrid Pennisetum

Publication date: Available online 12 September 2019Source: Biotechnology ReportsAuthor(s): Juanzi Wu, Nehal Kamal, Huanhuan Hao, Zhiwei Liu, Chen Qian, Yuhe Shao, Xiaoxian Zhong, Bin XuAbstractHybrid Pennisetum (Pennisetum americanum × P. purpureum Schumach L.) is considered as long, rapidly growing perennial C4 bunchgrasses and promising plant for heavy metals phytoremediation due to its high biomass productivity, high resistance to environmental stress, pests and diseases. Metal bioavailability level is the most important parameters to measure efficiency of phytoremediation. Recently, in order to improve metal accumulation of plant, the endophytic bacteria such as Bacillus megaterium were widely used on enhancing heavy metals phytoremediation through bioaccumulation or bioadsorption process. In the present study, the endophytic Bacillus megaterium wild type BM18 isolated from Hybrid Pennisetum was firstly delivered for 1 week exposure to different concentration of CdCl2.5/2 H2O ranging from 10 to 70 µM to obtain mutated strain BM18-2. After that, both the original and mutated strain was examined for their Cd accumulation ability, PGP (plant growth promoting) traits, improving Cd tolerance and plant growth of Hybrid Pennisetum. Moreover, the molecular mechanisms implicated in Cd bioremediation were further investigated. The results indicated higher Cd removal ability of mutated strain BM18-2 reached 52.7% compared with the original strain BM18 8.6%. Compared with BM18, th...
Source: Biotechnology Reports - Category: Biotechnology Source Type: research