Cholinergic system and exploratory behavior are changed after weekly-binge ethanol exposure in zebrafish

Publication date: Available online 6 September 2019Source: Pharmacology Biochemistry and BehaviorAuthor(s): Henrique Teza Bernardo, Jotele Fontana Agostini, Helena Cristina Zuehl Dal Toé, Karine Medeiros Vieira, Samira Leila Baldin, Patrícia Fernanda Schuck, Carolina Uribe-Cruz, Larisse Longo, Themis Reverbel da Silveira, Denis Broock Rosemberg, Eduardo Pacheco RicoAbstractBinge drinking is characterized by excessive alcohol consumption in a short period of time and is associated with a poor quality of life. Zebrafish are commonly used to investigate neurochemical, behavioral, and genetic parameters associated with ethanol (EtOH) exposure. However, few studies have used zebrafish as a model to investigate binge EtOH exposure. In order to elucidate the potential neurobehavioral impairments evoked by binge EtOH exposure in zebrafish, animals were immersed in 1.4% EtOH for 30 min three consecutive times with intervals of one week. Neurobehavioral parameters were analyzed immediately following the third exposure, as well as 2 and 9 days later. Brain choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities were reduced 9 days after the treatment. Thiobarbituric acid-reactive species and dichlorodihydrofluorescein levels were increased immediately after the treatment, but both returned to normal levels 2 days after the treatment. Catalase and glutathione reductase were impaired 2 and 9 days after the treatment. No alteration was observed in superoxide...
Source: Pharmacology Biochemistry and Behavior - Category: Biochemistry Source Type: research