Comparative transcriptome analysis reveals the signal proteins and defence genes conferring foot rot (Phytophthora capsici sp. nov.) resistance in black pepper (Piper nigrum L.)

This study had the objective to identify the candidate genes involved in the foot rot field tolerance of black pepper through comparative transcriptome analysis. P. capsici tolerant/moderately resistant cultivar IISR-Shakthi and susceptible cultivar IISR-Subhakara were artificially inoculated under controlled conditions and mRNAs were isolated from the leaves of treated and control plants at 0, 2, 4, 6, 12, 24, 48 h after inoculation. Transcriptome profiles were compared using DD-RT PCR on cDNA fragments and differentially expressed bands were sequenced. Sequence analysis revealed the involvement of signal proteins and defence enzymes Premnaspirodiene oxygenase, Phosphatase 2C-like domain-containing protein, Nitrous oxide reductase family maturation protein, putative disease resistance protein RGA3, Aspartyl protease, beta-glucosidase enzyme, Cytochrome P450 signal protein, Serine/Threonine Protein kinase WAG1 and nucleoredoxin 1-1 enzyme in field tolerance of black pepper to foot rot. Pathway analysis followed had paved additional insights in to the molecular mechanism of tolerance.Graphical abstract
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research