VivienLauYW, Maria Wong - Mutation and Expression Analysis of PTEN in Non-small Cell

VivienLauYW – The University of Hong Kong Medical Sciences Pathology Pharmacology MMedSc Mutation and Expression Analysis of PTEN in Non-small Cell Lung Cancer From Non-smokers Abstract This MMedSc dissertation studied the genetic variations and epigenetic variations of PTEN (phosphatase and tensin homolog deleted on chromosome 10) in relation to Adenocarcinoma (AD) in particular the protein expression level of PTEN in non-smoking non-small cell lung cancer (NSCLC) patient. PTEN gene is a tumor suppressor gene (TSG) residing at the locus10q23.3. A decrease in PTEN protein level induces the accumulation of lipid phosphoinosital 3,4,5-triphosphate (PIP3) and cell proliferation. Samples of 121 NSCLC patients were subjected to immunohistocheistry staining ro determine the association between PTEN protein expression level and clinicopathological feature of NSCLC. Out of the 121 NSCLC samples, 32 samples with no mutation in other known oncogenes such as EGFR and KRAS were screened for PTEN protein expression level, PTEN promoter hypermethylation and PTEN gene mutation. Direct sequencing on PTEN gene and methylation-specific PCR (MSP) were used as a detection method in this study. Wild type EGFR expression was related or a loss or reduced PTEN expression in NSCLC patient (P=0.006), and PTEN promoter non-methylation was associated with AD in NSCLC patients. Supervisor: Doctor Maria Wong
Source: New Media Medicine - Category: Universities & Medical Training Authors: Tags: St George's, University of London Source Type: forums