The N-terminal region of two-pore channel 1 regulates trafficking and activation by NAADP

Two-pore channels (TPCs) are NAADP-sensitive Ca2+-permeable ion channels expressed on acidic organelles. Here we show that deletion of the N-terminal region redirects TPC1 to the ER. Introduction of fluorophores at the N-terminus of TPC1 did not affect sub-cellular location but reversibly abolishes NAADP sensitivity. Our data reveal a dual role for the N-terminus in localization and function of TPC1

Source: BJ Cell - May 2, 2013 Category: Biochemistry Authors: D Churamani, R Hooper, T Rahman, E Brailoiu, S Patel Tags: BJ Signal Source Type: research