Studying Extracellular Signaling Utilizing a Glycoproteomic Approach: Lectin Blot Surveys, a First and Important Step
Successful innovative proteomic analysis is highly dependent on molecular biology techniques at the surveying and validation stage. This is because mass spectrometry (MS) analyses of complex samples are limited by their dynamic range for detection—so careful front-end sample preparation, fractionation, and enrichment are crucial to find biologically relevant signals in an extremely complex extracellular environment. Here, we share a very useful and simple front-end surveying methodology—lectin blotting—for proteomic analysis of glycosylation patterns—the most abundant posttranslational modification in extracellular signaling.
Source: Springer protocols feed by Biochemistry - Category: Biochemistry Source Type: news