Studying Extracellular Signaling Utilizing a Glycoproteomic Approach: Lectin Blot Surveys, a First and Important Step

Successful innovative proteomic analysis is highly dependent on molecular biology techniques at the ­surveying and validation stage. This is because mass spectrometry (MS) analyses of complex samples are limited by their dynamic range for detection—so careful front-end sample preparation, fractionation, and enrichment are crucial to find biologically relevant signals in an extremely complex extracellular environment. Here, we share a very useful and simple front-end surveying methodology—lectin blotting—for proteomic analysis of glycosylation patterns—the most abundant posttranslational modification in extracellular signaling.
Source: Springer protocols feed by Biochemistry - Category: Biochemistry Source Type: news