Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations

We present methods for the preparation of mouse ear sheets and their use in multidimensional confocal imaging experiments to monitor and analyze the directional migration of fluorescently labelled DCs through the dermis and into afferent lymphatic vessels. The assay provides a more physiological approach to study leukocyte migration than in vitro three-dimensional (3D) or 2-dimensional (2D) migration assays such as collagen gels and transwell assays.
Source: Springer protocols feed by Biochemistry - Category: Biochemistry Source Type: news