Construction of a chimeric thermoacidophilic beta-endoglucanase

Conclusions: The refolded active chimeric enzyme shows a temperature optimum of approximately 85[degree sign]C and a pH optimum of approximately pH 3 thus retaining the advantageous properties of the Sulfolobus parent enzyme. This study suggests that the targeted construction of chimeric enzymes is an alternative to point mutational engineering efforts as long as parent enzymes with the wanted properties are available.
Source: BMC Biochemistry - Category: Biochemistry Authors: Source Type: research