Serum concentration of homocysteine in spontaneous feline chronic kidney disease

Publication date: Available online 22 August 2019Source: The Veterinary JournalAuthor(s): M. Giraldi, S. Paltrinieri, C. Curcio, P. ScarpaAbstractSerum homocysteine (Hcy) increases in people and dogs with chronic kidney disease (CKD). Hyperhomocysteinemia (HHcy) has also been associated with CKD-related hypertension and proteinuria. The aims of this study were to: (1) validate an enzymatic method for quantification of Hcy in feline serum; (2) evaluate whether HHcy was associated with the presence and severity of CKD, proteinuria or hypertension; and (3) determine whether HHcy could predict disease progression.The intra- and inter-assay coefficients of variation (CVs) and the recovery rates of linearity under dilution and spiking recovery tests of the enzymatic method were 3.1–6.7%, 11.6–12.5%, 96.9 ± 5.4% and 96.9 ± 5.4%, respectively. Healthy cats at risk of CKD (n = 17) and cats with CKD (n = 19) were sampled over a 6-month period (63 samples in total). Cats with CKD had significantly higher Hcy concentrations (P = 0.005) than cats at risk. The concentration of Hcy was higher (P = 0.002) in moderate-severe CKD than in mild CKD and correlated moderately with serum creatinine (P < 0.0001; r = 0.51). The concentration of Hcy increased with the magnitude of proteinuria and correlated weakly with urinary protein to creatinine ratio (P = 0.045; r = 0.26). HHcy was not associated with hypertension. At the time of enrollment, Hcy co...
Source: The Veterinary Journal - Category: Veterinary Research Source Type: research