HER2/neu estimation in breast cancer: a comparative study between quantitative real-time PCR and immunohistochemistry

Conclusion: The study showed an overall moderate agreement between IHC and qRT-PCR. The low agreement between IHC and qRT-PCR in the positive cases was masked by the high agreement between both techniques in the negative cases. Together, our results support the unsuitable sole use of qRT-PCR as an alternative method for the detection of HER2/neu gene amplification in breast cancer.
Source: Egyptian Journal of Pathology - Category: Pathology Tags: Original Articles Source Type: research