In-situ immune profile of polymorphic vs. macular Indian Post Kala-azar Dermal Leishmaniasis

Publication date: Available online 22 August 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Ritika Sengupta, Shibabrata Mukherjee, Srija Moulik, Sneha Mitra, Surya Jyati Chaudhuri, Nilay Kanti Das, Uttara Chatterjee, Mitali ChatterjeeAbstractPost Kala-azar Dermal Leishmaniasis (PKDL), a sequel of apparently cured Visceral Leishmaniasis presents in South Asia with papulonodular (polymorphic) or hypomelanotic lesions (macular). Till date, the polymorphic variant was considered predominant, constituting 85–90%. However, following active-case surveillance, the proportion of macular PKDL has increased substantially to nearly 50%, necessitating an in-depth analysis of this variant. Accordingly, this study aimed to delineate the cellular infiltrate in macular vis-à-vis polymorphic PKDL. To study the overall histopathology, haematoxylin and eosin staining was performed on lesional sections and phenotyping by immunohistochemistry done in terms of dendritic cells (CD1a), macrophages (CD68), HLA-DR, T-cells (CD8, CD4), B-cells (CD20) and Ki67 along with assessment of the status of circulating homing markers CCL2, CCL7 and CXCL13. In polymorphic cases (n = 20), the cellular infiltration was substantial, whereas in macular lesions (n = 20) it was mild and patchy with relative sparing of the reticular dermis. Although parasite DNA was identified in both variants by ITS-1PCR, the parasite load was significantly higher in the polymorphic va...
Source: International Journal for Parasitology: Drugs and Drug Resistance - Category: Parasitology Source Type: research