Recent Developments in Novel Blue/Green/Red/NIR Small Fluorescent Probes for In Cellulo Tracking of RNA/DNA G-Quadruplexes

Publication date: Available online 11 August 2019Source: Journal of Photochemistry and Photobiology C: Photochemistry ReviewsAuthor(s): Anup Pandith, Ravikumara Guralamatta Siddappa, Young-Jun SeoAbstractThe detection and stabilization of G-quadruplexes (G4s) in living systems is of enormous applicability in the fields of chemical biology and therapeutic materials. Whereas DNA serves as a genetic material, RNA functions in the regulation and expression of genetic materials. Even there is various report on fluorescent probes invitro G4s recognitions, in this review we highlighted briefly, in-cellulo identification of G4s along with conventional methods principles. Although there are varieties of G4-forming sequences in the genome, targeting a specific type in living cells is currently not possible because of the high instability of G4s in cellular/subcellular systems. In contrast, several reports describe the in vitro identification of G4s, along with in-cell demonstrations, using efficient fluorescent probes, through either intrinsic or extrinsic approaches. In the intrinsic mode, the sensing results from the use of highly selective synthetic fluorescent oligonucleotides or proteins (a labeling approach). In the extrinsic mode, quencher-free small molecular probes are used to recognize specific G4s under physiological conditions. Because of their robustness, simplicity, and ease of handling, this review describes recent trends in the use of blue/green, green, red, and near-in...
Source: Journal of Photochemistry and Photobiology C: Photochemistry Reviews - Category: Chemistry Source Type: research