Two-Electrode Voltage Clamp

Two-electrode voltage clamp (TEVC) is a conventional electrophysiological technique used to artificially control the membrane potential (V
m) of large cells to study the properties of electrogenic membrane proteins, especially ion channels. It makes use of two intracellular electrodes—a voltage electrode as V
m sensor and a current electrode for current injection to adjust the V
m, thus setting the membrane potential at desired values and recording the membrane current to analyze ion channel activities. Here we describe the use of TEVC in combination with exogenous mRNA expression in Xenopus oocytes for ion channel recording.

Source: Springer protocols feed by Cell Biology - April 12, 2013 Category: Cytology Source Type: news