Micropipette Aspiration for Studying Cellular Mechanosensory Responses and Mechanics

Micropipette aspiration (MPA) is a widely applied method for studying cortical tension and deformability. Based on simple hydrostatic principles, this assay allows the application of a specific magnitude of mechanical stress on cells. This powerful method has revealed insights about cell mechanics and mechanosensing, not only in Dictyostelium discoideum but also in other cell types. In this chapter, we present how to set up a micropipette aspiration system and the experimental procedures for determining cortical tension and mechanosensory responses.

Source: Springer protocols feed by Microbiology - March 6, 2013 Category: Microbiology Source Type: news