Transcript Localization in Dictyostelium discoideum Cells by RNA FISH

We describe methods for the generation of suitable fluorescently labeled probes and the fixation of cells, by which we have localized different precursor and mature rRNA molecules to the nucleolus or the cytoplasm, respectively.

Source: Springer protocols feed by Microbiology - March 6, 2013 Category: Microbiology Source Type: news