Improved chlamydospore formation in Tricholoma bakamatsutake with addition of amino acids in vitro

Publication date: Available online 11 June 2019Source: MycoscienceAuthor(s): Takashi Yamanaka, Miki Konno, Masataka Kawai, Yuko Ota, Noritaka Nakamura, Akira OhtaAbstractTricholoma bakamatsutake, which forms its fruit body in oak forests, is similar to T. matsutake in shape and odor; therefore, it is expected to have a high economic value similar to T. matsutake. Recently, the fruit bodies of T. bakamatsutake were produced after planting of a Quercus phillyraeoides sapling with a pure culture of the fungal mycelia. This fungus forms pigmented thick-walled spores (chlamydospores [CSs]) terminally on the hyphae in culture and in the field settings. Abundant CS production in vitro seems to be advantageous for cultivating mycorrhizal trees inoculated with T. bakamatsutake CSs. We investigated the effects of adding 16 different nitrogen (N) compounds (three inorganic and 13 organic) to a culture medium on vegetative hyphal growth and CS formation. The growth of T. bakamatsutake was reduced by many of the N sources used. Conversely, the rate of CS formation was improved by the addition of valine or glutamine.

Source: Mycoscience - July 16, 2019 Category: Biology Source Type: research