Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics

Imaging molecularly defined regions of chromatin in single living cells during transcriptional activation has the potential to provide new insight into gene regulatory mechanisms. Here, we describe a method for isolating cell lines with multi-copy arrays of reporter transgenes, which can be used for real-time high-resolution imaging of transcriptional activation dynamics in single cells.

Source: Springer protocols feed by Genetics/Genomics - February 25, 2013 Category: Genetics & Stem Cells Source Type: news