Genome-wide identification and expression profiling of odorant-binding proteins in the oriental fruit fly, Bactrocera dorsalis

In this study, we expanded the repository of B. dorsalis OBPs to 49. Phylogenetic analysis of BdorOBPs with other species revealed that these proteins grouped into four subfamilies. Furthermore, we determined the expression profiles in six body parts (namely, the legs, wing, antenna, cuticles of the head, thorax, and abdomen) and five internal tissues (namely, the fat body, midgut, Malpighian tubule, testes, and ovary). The results indicated that 21 BdorOBPs showed high expression levels in the antenna, legs, and head cuticles and may thus perform olfactory functions, which corroborates previous evidence. Two BdorOBPs were specifically expressed in the abdomen cuticles. Nineteen OBPs were highly expressed in the fat body, while four OBPs were highly expressed in the reproductive organs. This indicated that they may have physiological roles other than in chemoreception. In summary, our results contribute to the knowledge base of insect OBPs and provide a foundation for the further study of the molecular mechanisms of chemoreception in B. dorsalis.Graphical abstract
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Category: Genetics & Stem Cells Source Type: research