Constant domain-exchanged Fab enables specific light chain pairing in heterodimeric bispecific SEED-antibodies

ConclusionIncorporation of a constant domain-exchanged Fab fragment into a SEED antibody yields functional molecules with favorable biophysical properties.General significanceOur results show that the novel engineered bispecific SEED antibody scaffold with an incorporated Fab fragment with CH3-exchanged constant domains is a promising tool for the generation of complete heterodimeric bispecific antibodies with correct light chain pairing.Graphical abstract

Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - July 9, 2019 Category: Biochemistry Source Type: research