Temporal expression of candidate genes at the Co-1 locus and their interaction with other defense related genes in common bean

Publication date: Available online 6 July 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Mahiya Farooq, Bilal A. Padder, Nadiem N. Bhat, M.D. Shah, Asif B. Shikari, Halima E. Awale, James D. KellyAbstractThe presence of multiple resistance genes in common bean and the diverse race spectrum of Colletotrichum lindemuthianum suggests that a broad spectrum of resistance mechanisms needs to be deployed in resistance breeding. However, limited studies on the interaction of anthracnose resistance genes and other plant defense genes exist in common bean. Therefore, we analyzed four candidate genes of Co-12 allele at the Co-1 locus and plant defense genes via quantitative real time PCR, to determine the fine-tuned timing and magnitude of expression of the resistance reaction. In the present study, a pair of near isogenic lines (NILs; T9576R and T9576S) that differ for the Co-12 resistance allele, was used that upon infection with race 73 of C. lindemuthianum show differential gene expression between the NILs. Our aim was to identify a particular gene that is effective against race 73 among other candidate genes located at the Co-1 locus. Gene expression analysis revealed higher expression levels of the Phvul.001G243800 gene. Expression was nearly 144 fold higher in the resistant NIL, than in the susceptible NIL and could be a possible candidate gene effective against race 73. In addition, we found that initially the other defense genes are activated which suggests t...
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research