13C-Based Metabolic Flux Analysis: Fundamentals and Practice

Isotope-based metabolic flux analysis is one of the emerging technologies applied to system level metabolic phenotype characterization in metabolic engineering. Among the developed approaches, 13C-based metabolic flux analysis has been established as a standard tool and has been widely applied to quantitative pathway characterization of diverse biological systems. To implement 13C-based metabolic flux analysis in practice, comprehending the underlying mathematical and computational modeling fundamentals is of importance along with carefully conducted experiments and analytical measurements. Such knowledge is also crucial when designing 13C-labeling experiments and properly acquiring key data sets essential for in vivo flux analysis implementation.

Source: Springer protocols feed by Biotechnology - February 19, 2013 Category: Biotechnology Source Type: news