Pretreatment of Populus tomentiglandulosa protects hippocampal CA1 pyramidal neurons from ischemia-reperfusion injury in gerbils via increasing SODs expressions and maintaining BDNF and IGF-I expressions

Publication date: June 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 6Author(s): Tae-Kyeong Lee, Joon Ha Park, Ji Hyeon Ahn, Hyunjung Kim, Minah Song, Jae-Chul Lee, Jong Dai Kim, Yong Hwan Jeon, Jung Hoon Choi, Choong Hyun Lee, In Koo Hwang, Bing-Chun YAN, Moo-Ho Won, Il Jun KangAbstractTo examine the effects of Populus tomentiglandulosa (PT) extract on the expressions of antioxidant enzymes and neurotrophic factors in the cornu ammonis 1 (CA1) region of the hippocampus at 5 min after inducing transient global cerebral ischemia (TGCI) in gerbils, TGCI was induced by occlusion of common carotid arteries for 5 min. Before ischemic surgery, 200 mg·kg−1 PT extract was orally administrated once daily for 7 d. We performed neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B staining. Furthermore, we determined in situ production of superoxide anion radical, expression levels of SOD1 and SOD2 as antioxidant enzymes and brain-derived neurotrophic factor (BDNF) and insulin-like growth factor I (IGF-I) as neurotrophic factors. Pretreatment with 200 mg·kg−1 PT extract prevented neuronal death (loss). Furthermore, pretreatment with 200 mg·kg−1 PT extract significantly inhibited the production of superoxide anion radical, increased expressions of SODs and maintained expressions of BDNF and IGF-I. Such increased expressions of SODs were maintained in the neurons after IRI. In summary, pretreated PT extract can significantly increase levels of SODs ...
Source: Chinese Journal of Natural Medicines - Category: Complementary Medicine Source Type: research