Genomic analysis and lactose transporter expression in Kluyveromyces marxianus CCT 7735

Publication date: Available online 15 June 2019Source: Fungal BiologyAuthor(s): Lílian Cardoso de Paiva, Raphael Hermano Santos Diniz, Pedro Marcus Pereira Vidigal, Tiago Antônio Oliveira Mendes, Mateus Ferreira Santana, María-Esperanza Cerdán, María-Isabel González-Siso, Wendel Batista da SilveiraAbstractKluyveromyces marxianus CCT 7735 has been used to produce ethanol, aromatic compounds, enzymes and heterologous proteins besides assimilates lactose as carbon source. Its genome has 10.7 Mb and encodes 4,787 genes distributed in 8 nuclear chromosomes and one mitochondrial. Contrary to K. lactis, which has a unique LAC12 gene (encodes lactose permease), K. marxianus possesses four. The presence of degenerated copies and Solo-LTRs related to retrotransposon TKM close to the LAC12 genes in K. marxianus indicates ectopic recombinations. The Lac12 permeases of K. marxianus and K. lactis are conserved, however the conservation is higher between the copy of the left side of the chromosome three and the unique copy of K. lactis, indicating that this copy is the ancestor. The expression of the four LAC12 genes occurred in aerobiosis and hypoxia. Notably, the high lactose consumption in hypoxia seems to be related to the high expression of the LAC12 genes.

Source: Fungal Biology - June 16, 2019 Category: Biology Source Type: research