Candidate genes involved in spiroacetal biosynthesis in the oriental fruit fly, Bactrocera dorsalis

Publication date: Available online 8 June 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Zhong-Zhen Wu, He Zhang, Meng-Qiu Qu, Yang Cui, Ming-Shun Chen, Shu-Ying Bin, Jin-Tian LinAbstractSpiroacetals are widespread in nature as components of volatile semiochemical secretions from many insect species. The general pathway for spiroacetal biosynthesis in Bactrocera sp. is preliminarily established, but many genes involved in this pathway remain to be characterized. By analyzing transcriptomes of the rectal glands (RGs) from immature and mature females of the oriental fruit fly, Bactrocera dorsalis, a set of genes encoding two acetyl-CoA carboxylases (ACCs), two fatty acid synthases (FASs), eight desaturases (DESs), twelve fatty acyl-CoA reductases (FARs), seventy-two cytochrome P450 enzymes (CYPs), and twenty-three odorant binding proteins (OBPs) were identified. We investigated the expression of candidate genes in immature and mature stages based on the RNA-seq data and Real-time quantitative PCR. Expression profiling revealed that some of these genes were primarily expressed in female rectal glands among different tissues, and were up-regulated in mature females. Semi-quantitative RT-PCR assays were also adapted to examine tissue-specific expression of selected candidate genes. Additionally, their putative functions in spiroacetal synthesis and transportation are proposed. Our study provided large-scale sequence information for fu...

Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - June 9, 2019 Category: Genetics & Stem Cells Source Type: research