DNA sequencing in high-throughput neuroanatomy

Publication date: Available online 4 June 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Justus M KebschullAbstractMapping brain connectivity at single cell resolution is critical for understanding brain structure. For decades, such mapping has been principally approached with microscopy techniques, aiming to visualize neurons and their connections. However, these techniques are very labor intensive and do not scale well to the complexity of mammalian brains. We recently leveraged the speed and parallelization of DNA sequencing to map the projections of thousands of single neurons in single experiments, and to map cortical mesoscale connectivity in single mice. Here, I review the state of sequencing-based neuroanatomy, and discuss future directions in synaptic connectivity mapping and comparative connectomics.
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research