Murine Whole-Organ Immune Cell Populations Revealed by Multi-epitope-Ligand Cartography

In this study, we adapted this method to identify a large panel of murine leukocyte subpopulations in a whole frozen section of a peripheral lymph node. Using the resulting antibody library, we examined non-inflamed versus inflamed tissues of brain and spinal cord in the experimental autoimmune encephalomyelitis (EAE) model. The presence and activity of specific leukocyte subpopulations (different T cell subpopulations, dendritic cells, macrophages, etc.) could be assessed and the cellular localizations and the corresponding activation status in situ were investigated. The results were then correlated with quantitative RT-PCR.

Source: Journal of Histochemistry and Cytochemistry - January 26, 2013 Category: Biochemistry Authors: Eckhardt, J., Ostalecki, C., Kuczera, K., Schuler, G., Pommer, A. J., Lechmann, M. Tags: Articles Source Type: research