Acid detoxified Inaba LPS (pmLPS) is a superior cholera conjugate vaccine immunogen than hydrazine detoxified LPS and induces vibriocidal and protective antibodies

Abstract Worldwide, in endemic areas of cholera, the group most burdened with cholera is children. This is especially vexing as young children (2‐5 years old) do not respond as well, or for as long as adults do to the current killed, oral cholera vaccines (OCV). Conjugate vaccines based on the hapten‐carrier paradigm have been developed for several bacterial pathogens that cause widespread and severe diseases in young children. We and others have studied different formulations of Vibrio cholerae (Vc) O1 lipopolysaccharide (LPS, a T independent antigen) conjugates. Detoxified LPS is a central component of a LPS‐based conjugate vaccine. pmLPS which is detoxified by acid treatment is a superior immunogen compared to hydrazine detoxified LPS (DetAcLPS) that has altered lipid A acyl chains. The other feature of pmLPS is the ability to link carrier proteins to a core region sugar. pmLPS readily induced vibriocidal antibodies following one intraperitoneal dose in a MPL type adjuvant One dose of the pmLPS conjugate was suggestive of being protective; a booster resulted in protective antibodies for infant mice challenged with virulent cholera. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
Source: FEMS Immunology and Medical Microbiology - Category: Microbiology Authors: Tags: Research Paper Source Type: research