Making standards for quantitative real-time pneumococcal pcr

Publication date: Available online 4 December 2014 Source:Biomolecular Detection and Quantification Author(s): Susan C. Morpeth , Jim F. Huggett , David R. Murdoch , J. Anthony G. Scott Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research