In vitro removal of deoxynivalenol by a mixture of organic and inorganic adsorbents

The objective of this study was to evaluate the efficacy of a mixture of inorganic (activated carbon) and organic (yeast cell wall) adsorbents on in vitro removal of deoxynivalenol (DON). The study was carried out using a 24 incomplete factorial design with three replications at the central point, totalling 11 experiments. The independent variables were pH (3.0, 5.0 and 7.0), adsorbent concentration (0.2, 1.1 and 2.0%), DON concentration (2,500, 5,000 and 7,500 ng/ml) and ratio of activated carbon and yeast cell wall (0:100, 15:85 and 30:70), evaluated at 30, 60 and 90 min incubation periods. The highest percentage of adsorption occurred with 2.0% activated carbon and yeast cell wall at 30:70 ratio (≯95.6%) for 30, 60 and 90 min. The lowest adsorption was detected using 0.2% of activated carbon and yeast cell wall at 0:100 ratio (from 14.4 to 77.3%). The pH values (3.0, 5.0 and 7.0) showed no influence on the adsorption of DON in vitro only at 2.0% inclusion level. The predictive model of integrated optimisation of the independent variables of in vitro DON adsorption describes that the maximum adsorption (100%) occurs when the variables pH and adsorbent concentration are set at +1 coded level (pH 7.0 and 2.0%, respectively) and the toxin concentration and the ratio of activated carbon and yeast cell wall at -1 coded level (2,500 ng/ml and 30:70, respectively) for 30, 60 and 90 min. Statistical analysis showed that the equation model obtained can be applied to predict the ad...
Source: World Mycotoxin Journal - Category: Toxicology Tags: World Mycotoxin Journal Source Type: research