Parameters Affecting the Accuracy of Nanoparticle Shape Measurement in 3D

Publication date: Available online 13 May 2019Source: MicronAuthor(s): Misa Hayashida, Kai Cui, Darren Homeniuk, Rinyaporn Phengchat, Arthur M. Blackburn, Marek MalacAbstractWhile electron tomography can be used to visualize objects at nanoscale, it is difficult to perform reproducible quantitative measurements. Here we measure the shape of nanoparticles (NPs) in three dimensions (3D) using electron tomography. We evaluated the accuracy of maximum Feret diameter (Feretmax), minimum Feret diameter (Feretmin) and volume of NPs measurements from reconstructed 3D images which were obtained from data acquired with varied electron dose. We perform both simulations and experiment to clarify what factors effect on the accuracy of the NP shape measurement. Based on the results, suitable threshold for binarization and reconstruction methods were evaluated. We also report comparison results obtained on exactly the same samples in two different laboratories.

Source: Micron - May 14, 2019 Category: Biology Source Type: research