In vivo protective effect of Rosmarinus officinalis oil against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats

This study aimed to explore the hepatoprotective effects of cold-pressed R. officinalis oil (CPRO) against CCl4-induced liver toxicity in the experimental rats. Fatty acids and bioactive lipids of CPRO were analyzed. CPRO was orally administered to rats in two doses (100 and 200 mg/kg) along with CCl4 (1 mL/kg in olive oil) for 8 weeks. Indices of liver and kidney functions, lipid profile and oxidation were evaluated in rats’ serum and tissues. In CPRO the percentages of polyunsaturated, monounsaturated, and saturated fatty acids were 42.3%, 41.7%, and 15.8%, respectively. CPRO contained high amounts of total phenolic compounds (7.20 mg GAE/g). α-, β-, γ- and δ-tocotrienols accounted for 18, 12, 29, and 158 mg/100 g CPRO, respectively, while α-, β-, γ- and δ-tocopherols accounted for 291, 22, 1145, and 41 mg/100 g CPRO, respectively. The LD50 at 24 h was 5780 mg/kg. Treatment with 200 mg/kg CPRO caused a decrease in creatinine, urea and uric acid levels to 0.66, 28.3 and 3.42 mg/dL, respectively. After 8 weeks of administration, levels of total lipids (TL), total cholesterol (TC), total triacylglycerol (TAG), low dentistry lipoprotein-cholesterol (LDL-C) and very low dentistry lipoprotein-cholesterol (VLDL-C) were decreased to 565, 165, 192, 75.6 and 38.5 mg/L, respectively. Malondialdehyde levels in liver were reduced and glutathione levels were elevated in CPRO-treated rats. CPRO reduced the activity of ALT, AST, and ALP as well as kidney f...
Source: PharmaNutrition - Category: Nutrition Source Type: research