Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy

Confocal laser scanning microscopy (CLSM) is a useful research tool to explore the exopolysaccharides (EPS) in bacterial biofilms. Here, we describe the analysis of different biofilms of Myxococcus xanthusformed in a modified chamber slide system with CLSM. In conjunction with several specific fluorescent probes, the EPS within M. xanthusbiofilms can be visualized and analyzed in situ.

Source: Springer protocols feed by Microbiology - January 10, 2013 Category: Microbiology Source Type: news