Effect of Huazhuojiedu medicated serum on the proliferation and activation of hepatic stellate cells and the expression of PI3K and p-Akt in rats.

Effect of Huazhuojiedu medicated serum on the proliferation and activation of hepatic stellate cells and the expression of PI3K and p-Akt in rats. Int J Clin Exp Med. 2014;7(10):3280-3288 Authors: Kang L, Wang Y, Zhang M, Sun R, Lou Y, Wang Y, Li D Abstract To observe the effect of Huazhuojiedu medicated serum on the proliferation and activation of hepatic stellate cells, as well as the expression of PI3K and p-Akt in rats, and to explore the underlying mechanism of Huazhuojiedu prescription against hepatic fibrosis. Hepatic stellate cells harvested from rats were resuscitated and subcultured, followed by the intervention of Huazhuojiedu equivalent dose, Huazhuojiedu double dose, and positive drug (Compound Biejiaruangan Troche) medicated serum of rats. After in vitro culture, hepatic stellate cells were stimulated with 5 ng/mL transforming growth factor-β1. At 24, 48, 72 hours, the proliferation of hepatic stellate cells was detected with MTT assay; at 48 hours, α-SMA mRNA and protein expression in hepatic stellate cells were determined with RT-PCR assay and western blot analysis, respectively, to evaluate the activation of hepatic stellate cells; in addition, PI3K and p-Akt protein expression levels were also assayed with western blot analysis at 48 hours. The results showed that, 24-hour transforming growth factor-β1 stimulation significantly promoted the proliferation of hepatic stellate cells (P < 0.01). Each medicated ser...
Source: International Journal of Clinical and Experimental Medicine - Category: Drugs & Pharmacology Tags: Int J Clin Exp Med Source Type: research