Quantitating fatty acids in dried blood spots on a common collection card versus a novel wicking sampling device

Publication date: Available online 2 May 2019Source: Prostaglandins, Leukotrienes and Essential Fatty AcidsAuthor(s): Jan Gunash, Juan J. Aristizabal-Henao, Ken D. StarkAbstractBlood biomarkers of n-3 polyunsaturated fatty acids can serve as indicators of dietary intake and benefits and/or disease risk. The use of dried blood spots for fatty acid analyses is increasing but most of the reported data is qualitative (relative percentages of total fatty acids). The ability to quantitate concentrations of fatty acids on a common blood spot collection card and a novel wicking device designed to collect 10μL of blood was compared with a wet blood sample in ten young adult participants. Prior to this comparison, the collection materials were screened for contaminants by gas chromatography with flame ionization and liquid chromatography/tandem mass spectrometry, and the blood volume and blood spot area relationship of the collection card was confirmed using technical replicates. Palmitate and stearate were detected as free fatty acids on both collection materials and as lysophosphatidylcholines on the wicking device. The low amounts (<10ng) did not affect the quantitation of these fatty acids in either material. The relationship between blood volume and blood spot area was linear (r = 0.99, p <0.001) and it was determined that a 6mm hole punch contained 9.6 µL of blood. When compared with wet blood, the fatty acid determinations from the dried blood spots were largely si...

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - May 2, 2019 Category: Lipidology Source Type: research