Nanosized Selenium and Loranthus micranthus leaves ameliorate Streptozotocin-induced hepato-renal dysfunction in rats via enhancement of antioxidant system, regulation of Caspase 3 and Nrf2 protein expression

Publication date: Available online 1 May 2019Source: PharmaNutritionAuthor(s): Azubuike P. Ebokaiwe, Omamuyovwi M. Ijomone, Sharoon Griffin, Richard C. Ehiri, Kebe E. Obeten, Joseph O. Nw.wo, Chukwunonso E.C.C. Ejike, Cornelia M. KeckAbstractThe investigation of potential health benefits of nano-sized nutriceuticals is recently a major scientific interest. The present study investigated the effect of Selenium and Loranthus micranthus leaves nanoparticles (SeNPs and LMLNPs), on streptozotocin (STZ)-diabetes induced hepato-renal dysfunction in rats. Adult rats were rendered diabetic by a single i.p exposure to 40 mg/kg STZ. Thereafter, rats were treated with 0.1 mg/kg SeNPs or 200 mg/kg LMLNPs, or a combination of both, while 50 mg/kg metformin was used as a standard therapeutic drug. Results indicated that STZ-diabetic rats experienced liver and kidney damage evidenced by a significant elevation in serum activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and 5' nucleotidase (5'NT) when compared to non-diabetic control rats. Furthermore, a decrease in activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), Glutathione-S-transferase (GST) and glutathione (GSH) levels was observed, whereas myeloperoxidase (MPO) activities and lipid peroxidation (LPO) levels increased significantly in the negative control group compared to the test groups. Immunohistochemical and western blot analyse...
Source: PharmaNutrition - Category: Nutrition Source Type: research