In vitro substrate phosphorylation by Ca2+/calmodulin-dependent protein kinase kinase using guanosine-5¿-triphosphate as a phosphate donor

Conclusions:
These results indicated that screens that use STO-609 as a CaMKK inhibitor and Mg-GTP as a CaMKK-dependent phosphate donor might be useful to identify previously unidentified downstream target substrates of CaMKK.

Source: BMC Biochemistry - December 5, 2012 Category: Biochemistry Authors: Saki YurimotoTomohito FujimotoMasaki MagariNaoki KanayamaRyoji KobayashiHiroshi Tokumitsu Source Type: research