Effect of an extraction solvent in the antioxidant quality of Pinus densiflora needle extract

Publication date: Available online 15 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Thamizhiniyan Venkatesan, Young-Woong Choi, Young-Kyoon KimAbstractPinus densiflora needle extract (PDNE) is widely reported to have many pharmacological activities including an antioxidant. However, the solvent system used for extraction greatly affects its antioxidant quality. Hence, in the present study, we investigated the effect of a different ratio (volume/volume) of ethanol to water (0–100%) in the extraction of PDNE with potent antioxidant capacity. The chemical assays, 2,2-diphenyl-1 picrylhydrazyl (DPPH), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were conducted to assess the antioxidant potential of PDNE. Subsequently, the cytoprotective effect of PDNE was determined using tert-butyl hydroperoxide (TBHP)-challenged hepG2 cellular model. The needle extracts from 40% ethanol (PDNE-40) showed greater radical scavenging activity followed by 60, 20, 80, 0 and 100% ethanol extracts. EC50 value of the most active extract, PDNE-40 was 8.56 ± 0.51 μg/mL, relative to 1.34 ± 0.28 μg/mL of the standard trolox (for ABTS radical), and 75.96 ± 11.60 μg/mL, relative to 4.83 ± 0.26 μg/mL of the standard trolox (for DPPH radical). Either PDNE-20 or PDNE-40 pretreatment remarkably decreased the levels of reactive oxygen species (ROS), lipid peroxides and protein carbonyls in TBHP-challenged HepG2 cells. In addi...
Source: Journal of Pharmaceutical Analysis - Category: Drugs & Pharmacology Source Type: research