Preparation and Measuring of Brain GABAA R-coupled Cl- /HCO3 - - Activity for Integral Assessment of Aquatic Toxicity.

In this study, we present a protocol for isolation of the membrane-bound Cl- /HCO3 - -ATPase from neuronal cells of animal brain. We then describe an uncomplicated in vitro method for measuring this ATPase activity for assessment of toxicity after interaction of this protein with an aquatic sample. This assay offers new avenues for using the Cl- /HCO3 - -ATPase as a biomarker of water toxicity. This biotest is efficient, requires very little of the enzyme, and retains its sensitivity at low levels of various compounds. © 2019 by John Wiley & Sons, Inc. PMID: 30843667 [PubMed - as supplied by publisher]
Source: Current Protocols in Toxicology - Category: Toxicology Tags: Curr Protoc Toxicol Source Type: research