Early α-linolenic acid exposure to embryo reduces pentylenetetrazol-induced seizures in zebrafish larva

Publication date: Available online 1 March 2019Source: Prostaglandins, Leukotrienes and Essential Fatty AcidsAuthor(s): Savita Kumari, Arindam Ghosh Mazumder, Avantika Bhardwaj, Damanpreet SinghABSTRACTOver the past few years, there has been a tremendous increase in interest of general population toward food-based therapies for management of chronic clinical conditions due to their lesser adverse effects with prolonged use over pharmacotherapies. Foods enriched with omega-3 fatty acids have shown some promising results in case of epilepsy. The present study was envisioned to investigate the effect of early exposure of α-linolenic acid (ALA), an essential omega-3 fatty acid in developing zebrafish (Danio rerio) embryos toward pentylenetetrazol (PTZ)-induced seizure susceptibility. The healthy wild-type zebrafish embryos were incubated in system water or system water containing different ALA concentrations (1-20 µM) till 7 dpf (days post fertilization). Each larva at 7 dpf was placed in 8 mM PTZ solution and seizures event was recorded. ALA incubation at 10 µM and 20 µM concentrations showed a dose-dependent reduction in PTZ-mediated hyperactive responses in larvae indicated by a marked decrease in total distance travelled and speed, as compared to vehicle control. Furthermore, both the treated groups showed increase in the latency to PTZ-induced clonus-like seizures in larvae, as compared to vehicle control. ALA incubated larvae at 10 µM and 20 µM concentrations also sho...

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - March 1, 2019 Category: Lipidology Source Type: research