Quantitative analysis of copy number variants based on real-time LightCycler PCR.

Quantitative analysis of copy number variants based on real-time LightCycler PCR.
Curr Protoc Hum Genet. 2014;80:Unit 7.21.
Authors: Ma L, Chung WK
Abstract
Quantitative real-time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. A simple method is described here to quantify DNA copy number from human samples.
PMID: 24510682 [PubMed - indexed for MEDLINE]

Source: Current Protocols in Human Genetics - November 25, 2014 Category: Genetics & Stem Cells Tags: Curr Protoc Hum Genet Source Type: research