Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti

Publication date: Available online 21 February 2019Source: Molecular and Biochemical ParasitologyAuthor(s): T. Basika, G.P. Paludo, F.M. Araujo, A.C. Salim, F. Pais, L. Maldonado, N. Macchiaroli, J. Camargo de Lima, M. Rosenzvit, G. Oliveira, L. Kamenetzky, H.B. FerreiraAbstractCestode development involves complex morphological and physiological changes. Here, we performed a differential expression analysis of gene transcripts between two developmental stages of the model cestode Mesocestoides corti. A RNA-seq-based approach was used to compare the transcriptomes of the tetrathyridium (larval, TT) and strobilated worm (ST) stages of the parasite. We found 19,053 transcripts, from which ˜45% were complete matches to genes previously annotated in the available M. corti draft genome sequence, ˜24% were considered novel isoforms, and ˜24% were considered potential novel transcripts. Stage-specific transcripts were found for both TTs (66) and STs (136), along with shared transcripts significantly overrepresented in one stage (342 in TTs, and 559 in STs). Differential expression and Gene Ontology term enrichment analyzes provided evidence of upregulation of different sets of transcripts associated with ‘cytoskeleton’, ‘metabolism’ and ‘oxidation-reduction’ processes in each stage, suggesting functional involvement of the corresponding genes with stage-specific features. Transcripts and processes enriched in the TT reflect typical larval processes that occur with the ...
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research