Production of Polyhydroxyalkanoates Copolymers by Recombinant < b > < i > Pseudomonas < /i > < /b > in Plasmid- and Antibiotic-Free Cultures

We reported for the first time the insertion of a chromosomal copy ofphaC using the transposon system mini-Tn7. Stable antibiotic marker-free and plasmid-free recombinants were obtained. Subsequently, P(3HB-co-3HAMCL) was produced by these recombinants using glucose as the sole carbon source, without the need for co-substrates and under antibiotic-free conditions. A recombinant harboringA. hydrophila phaC produced a terpolyester composed of 84.2 mol% of 3-hydroxybutyrate, 6.3 mol% of 3-hydroxyhexanoate, and 9.5 mol% of 3-hydroxydecanoate from only glucose. Hence, we were successful in increasing the industrial potential ofPseudomonas sp. LFM461 strain by producing PHA copolymers containing 3HB and 3HAMCL using an unrelated carbon source, for the first time in a plasmid- and antibiotic-free bioprocess.J Mol Microbiol Biotechnol 2018;28:225 –235
Source: Journal of Molecular Microbiology and Biotechnology - Category: Microbiology Source Type: research