Variations of 1-octen-3-ol and lipoxygenase gene expression in the oyster mushroom Pleurotus ostreatus according to fruiting body development, tissue specificity, maturity, and postharvest storage

This study investigated variations in 1-octen-3-ol and the LOX gene (Polox1 and Polox2) expression together with LOX activity in P. ostreatus, according to fruiting body development, tissue specificity, maturity, and postharvest storage. 1-Octen-3-ol content was abundant in the stipe of medium-sized fruiting bodies (40–50 mm cap diameter), but decreased after 2 d of storage at 4 °C. During fruiting body development, changes in LOX activity mimicked changes in transcript levels of Polox1, which was much more abundantly expressed than Polox2. The changes in LOX activity were different from changes in 1-octen-3-ol levels according to fruiting body development, maturity, and postharvest storage. Our investigation suggests that medium-sized fruiting bodies that were not subjected to long-term storage would have a good aroma quality and that there is no correlation between 1-octen-3-ol synthesis and LOX activity, which is largely dependent on Polox1 expression.
Source: Mycoscience - Category: Biology Source Type: research