Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge

The cross-reactivity of antibodies employed within immunochemistry-based analytical methods may lead to overestimation of the results. Under certain conditions, specifically when controlling mycotoxin maximum limits serious problems can be encountered. Not only the structurally related mycotoxins, such as their masked (conjugated) forms, but also the unidentified matrix components are responsible for concentration overestimation of respective target analytes. The cross-reactivity phenomenon may also pose a risk of miss-interpretation of the proficiency tests results, when the assigned value becomes influenced by over-estimated results reported by users of immunochemical tests. In this paper, the current state of the knowledge on trueness problems associated with the rapid screening immunochemical methods have been reviewed. Special attention is focused on discussion of cross-reactivity in the ELISA tests, because this rapid test dominates the routine screening practice. However, the cross-reactions reported in lateral flow test strips, fluorescence polarisation immunoassay, or immunosensors have also been addressed. Content Type Journal ArticleCategory Review-ArticlePages 449-464DOI 10.3920/WMJ2014.1701Authors M. Zachariasova, Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, Institute of Chemical Technology, Prague, Technicka 3, 166 28 Prague 6, Czech RepublicP. Cuhra, Czech Agriculture and Food Inspection Authority, Za Opravnou 300/6,...
Source: World Mycotoxin Journal - Category: Toxicology Tags: World Mycotoxin Journal Source Type: research