Solid-phase extraction using molecularly imprinted polymer for determination of ochratoxin A in human urine

A rapid, selective and reliable sample preparation technique employing solid-phase extraction (SPE) based on molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) in human urine was described. After sample adjustment to pH 2.5 with 0.1 M HCl, the urine sample was loaded onto the MIP-SPE column, and after a wash step, OTA was eluted for measurement by ultra-high performance liquid chromatography coupled with fluorescence detection. Key parameters which affected the MIP-SPE extraction efficiency were optimized as was the detection method. Under the optimised conditions, the limits of detection and quantification for OTA in urine were 0.2 ng/ml and 0.6 ng/ml, respectively. The recoveries for OTA in urine, spiked at the 0.6, 6.0 and 60 ng/ml levels, ranged from 92.0 to 98.9%. Sixty urine samples were analysed, of which four were found to contain OTA at concentrations ranging from 0.022 to 0.083 ng/ml; the positive results were confirmed by liquid chromatography coupled with tandem mass spectrometry. OTA determination in urine is a good indicator for human exposure to the mycotoxin, and this is the first report on OTA contamination in Chinese people.
Content Type Journal ArticleCategory Research-ArticlePages 1-7DOI 10.3920/WMJ2013.1633Authors
L. Xie, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant Development, Malianwa North Road 151, Beijing 100193, ChinaP. Sheng, School of Traditional Chinese Medicine, ...

Source: World Mycotoxin Journal - October 1, 2014 Category: Toxicology Tags: World Mycotoxin Journal Source Type: research