Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection

Genetically engineered rodents have been generated worldwide for biomedical research. Recently, gene targeting techniques have been developed by using engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without using embryonic stem (ES) cells. This chapter introduces the latest protocols for producing genetically modified rodents using ZFN, TALEN, and CRISPR/Cas9.

Source: Springer protocols feed by Genetics/Genomics - November 20, 2014 Category: Genetics & Stem Cells Source Type: news