Genome Engineering Using CRISPR-Cas9 System

We present all relevant methods including the initial site selection, molecular cloning, delivery of guide RNAs (gRNAs) and Cas9 into mammalian cells, verification of target cleavage, and assays for detecting genomic modification including indels and homologous recombination. These tools provide researchers with new instruments that accelerate both forward and reverse genetics efforts.

Source: Springer protocols feed by Genetics/Genomics - November 20, 2014 Category: Genetics & Stem Cells Source Type: news