Direct injection high performance liquid chromatography coupled to data independent acquisition mass spectrometry for the screening of antibiotics in honey

Publication date: Available online 29 January 2019Source: Journal of Food and Drug AnalysisAuthor(s): Annie von Eyken, Daniel Furlong, Samareh Arooni, Fred Butterworth, Jean-François Roy, Jerry Zweigenbaum, Stéphane BayenAbstractThe targeted analysis of veterinary drug residues in honey traditionally involves a series of extraction and purification steps prior to quantification with high performance liquid chromatography coupled to high resolution or tandem mass spectrometry. These steps, designed to separate the target analytes from interferences, are generally time-consuming and costly. In addition, traditional cleanup steps are likely to eliminate other compounds whose analysis could prove decisive in current or future assessment of the honey sample. Alternatively, direct injection without complex sample preparation steps has been introduced for the fast analysis of trace compounds in environmental and food matrices. The aim of this study was to develop a rapid method for the targeted analysis of 7 key veterinary drug residues in honey based on direct injection high performance liquid chromatography coupled to quadrupole time-of-flight, while simultaneously recording data-independent MS/MS (e.g. All Ions MS/MS data) for future re-examination of the data for other purposes. The new method allowed for the detection of the target residues at levels approximately 20–100 times lower than current regulatory limits, for a total analysis time of about 45 min. The recoveries (...
Source: Journal of Food and Drug Analysis - Category: Food Science Source Type: research