Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro

Publication date: Available online 24 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Djelić Ninoslav, Radaković Milena, Borozan Sunčica, Vesna Dimirijević-Srećković, Pajović Nevena, Vejnović Branislav, Borozan Nevena, Ezgi Eylül Bankoglu, Stopper Helga, Stanimirović ZoranAbstractDiabetes represents one of the major health concerns, especially in developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells (PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 µM) in comparison to PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress (TBARS, catalase, lactate dehydrogenase) were monitored. It was observed that higher concentrations of adrenaline (1 and 10 µM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research